TriColor Prestained Protein Ladder (10 ~ 180kDa; 10 bands), 502011

TriColor Prestained Protein Ladder (10 ~ 180kDa) is a ready-to-use protein marker supplied in a gel loading buffer. The ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. It is designed for monitoring protein separation during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, and for sizing of proteins. The ladder is supplied in protein gel loading buffer and is ready-to-use. No need to heat, dilute, or add a reducing agent before loading. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green brand at 25kDa and one red band at 70kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer).

Recommendations for Loading:
Load an appropriate volume of the ladder onto the gel (5ul per well at 0.751.0mm thick gel and 10ul per well at 1.5mm thick gel). 2~3ul per well for general Western transfer.