ECL-Plus Chemiluminescent Substrate kit- western blot detection
ECL-PLUS Chemiluminescent Substrate is an ultra-sensitive luminal-based enhanced chemiluminescence (ECL) substrate, directly or indirectly detects protein conjugated with horseradish peroxidase (HRP).
This unique luminescent substrate system is the most sensitive commercial fluorescent detection reagent, with high sensitivity and high signal-to-noise ratio, in which 10-100 FG trace antigens can be detected. With rapid luminescence, the X-ray film can be retained for more than 12 hours, particularly suitable for the detection of trace proteins or nucleic acids. At the same time, the reagent allows the use of a higher antibody dilution ratio (1:2000-1:10000), a very safe antibody, to maximize the savings of antibodies.
Recommended primary antibody concentration: 1:1,000–1:20,000 dilution (0.05–1 µg/ml)
Recommended secondary antibody concentration: 1:25,000–1:200,000 dilution (5–40 ng/ml).
| Size |
2 x 50ml / 2 x 250ml |
| Product Category |
Western-Blot Detection |
| Kit Components |
1. ECL-Plus Reagent-A (Luminol & Enhancer): 50ml/250ml
2. ECL-Plus Reagent-B (Peroxide & stabilizer): 50ml/250ml |
| Storage/Stability |
2 ~ 8°C/1 year |
| Shipping |
Ambient |
