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Principle
- Peptone Salt Diluent is composed of peptone and sodium chloride. Peptone serves as the simplest source of the nitrogen, carbon, and other growth factors such as vitamins.
- It contains peptone at a low concentration which provides nutrients for the survival of microorganisms and hence protects the organisms.
- Sodium chloride at 0.85% concentration maintains the osmotic balance of the medium thereby maintaining cell morphology and integrity.
- The pH of this diluent medium is near the neutral range optimum for the viability of microorganisms. It is recommended by ISO Committee (1) for use as an isotonic diluent and used as a diluent for carrying out dilutions of different samples.
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Use: Recommended by ISO Committee for use as an isotonic diluent for food samples and used as a diluent for carrying out dilutions of different samples.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Off-white to light Beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Pale yellow color clear solution
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pH
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7.0 ±0.2 at 25 ˚C (9.50 g/ l)
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