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Principle
- Yeast extract rose bengal broth base is composed of yeast extract, disodium phosphate, bile salts, sodium chloride, magnesium Sulphate, sodium pyruvate, and rose bengal.
- Yeast extract rose bengal broth is formulated as recommended in APHA for the enrichment of Yersinia species from foods. Yeast extract provides nitrogen, carbon, vitamins, amino acids, and other trace nutrients required for the growth of microorganisms.
- Bile salts are used as selective agents for the isolation of gram-negative microorganisms, inhibiting gram-positive cocci. Disodium phosphate acts as a buffering agent.
- Sodium chloride maintains the osmotic equilibrium of the medium. Magnesium sulfate provides ions for metabolic reactions. Sodium pyruvate increases the recovery of stressed cells and enhances the growth of Yersinia species. Rose Bengal is a selective agent, that inhibits bacterial growth.
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