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Principle
- Yeast mannitol broth is used for the cultivation of nitrogen-fixing Rhizobium. It is composed of yeast extract, mannitol, dipotassium phosphate, magnesium sulfate, sodium chloride, and calcium carbonate.
- Yeast extract serves as a good source of readily available amino acids, vitamin B complex, and accessory growth factors for Rhizobia.
- It also aids in the oxidation-reduction potential of the medium in the range favorable for Rhizobia and serves as a hydrogen donor in the respiratory process.
- Mannitol is the carbon source. Dipotassium phosphate resists the change in pH of the medium. Magnesium sulfate and calcium carbonate provide cations essential for the growth of Rhizobia.
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