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Principle
- Wilson Blair agar base is developed by Wilson and Blair (1926); the media is composed of special peptone, meat extract, dextrose, sodium chloride, and agar. Meat extract and peptone provide nitrogen and minerals.
- Dextrose is an energy source. Sodium chloride maintains osmatic equilibrium and agar is a solidifying agent. The media is fortified with sodium sulfite, disodium phosphate, bismuth ammonium citrate, and ferrous sulfate.
- Ferrous sulfate is used for the detection of hydrogen sulfide production. The hydrogen sulfide producers form a precipitate with ferrous sulfate giving brown to black color with a metallic sheen to the positive cultures.
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