Tryptic Soy Broth (mTSB) Modified Base Medium (Without Novobiocin)

 Principle

• Modified tryptone soya broth is composed of tryptone, soy peptone, dextrose, sodium chloride, dipotassium phosphate, and bile salts. Tryptone and soy peptone provide nitrogen and other essential nutrients.
• Dextrose is a carbon source and serves as an energy source for optimum growth. Sodium chloride maintains osmotic balance, while di-potassium phosphate is a buffering agent. Bile salts inhibit the gram-positive bacteria. The novobiocin can be added to increase the selectivity for Escherichia coli associated with food-borne illness, it inhibits associated microorganisms.

 Use: Recommended for selective cultivation and enrichment of a wide variety of Escherichia coli associated with food-borne illness as recommended by FDA-BAM.