Tryptic Soy Broth (mTSB) Modified Base Medium (With Novobiocin)

 Principle

• Modified tryptone soya broth is composed of tryptone, soy peptone, dextrose, sodium chloride, dipotassium phosphate, bile salts, and novobiocin.
• Tryptone and soy peptone provide nitrogen and other essential nutrients. Dextrose is a carbon source and serves as the energy source for optimum growth. Sodium chloride maintains osmotic balance, while di-potassium phosphate is a buffering agent. Bile salts inhibit gram-positive bacteria.
• The novobiocin is added to increase the selectivity for Escherichia coli associated with food-borne illness, it inhibits associated microorganisms.

 Use: Recommended for selective cultivation and enrichment of a wide variety of Escherichia coli associated with food-borne illness as recommended by FDA-BAM.