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Principle
- Tributyrin agar is used for the detection and enumeration of lipolytic microorganisms such as Staphylococci, Clostridia, and Pseudomonas and molds in foodstuffs and other materials. Tributyrin is the simplest triglyceride occurring in natural fats and oils.
- It is hydrolyzed by some microorganisms. The basal medium is composed of peptone, yeast extract, and agar. Peptone and yeast extract in the medium provide nutrients to the organisms. Agar is the solidifying agent.
- Tributyrin is added to the medium for the isolation of lipolytic microorganisms. Tributyrin degradation by the microorganisms is indicated by clear zones surrounded by lipolytic bacteria.
- Lipolytic organisms turn the medium transparent by converting the fat to water soluble butyric acid.
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Use: Recommended for isolation and detection of lipolytic microorganisms.
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Beige colored powder.
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Solution
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Solubility
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In Soluble in Distilled water at 50˚ C
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Color
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Light amber colored opalescent
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pH
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7.5 ±0.2 at 25 ˚C (23.00 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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Light amber colored opalescent gel with tributyrin drop lets.
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