TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic technique that uses traditional chemical mutagenesis methods to create libraries of mutagenized individuals that are later subjected to high-throughput screens for the discovery of mutations. Previous TILLING protocols employed enzymatic or physical discrimination of heteroduplexed from homoduplexed target DNA. But these methods are very laborious, time-consuming, need extensive automation and sometimes have lower sensitivity and resolution than needed and the heteroduplexes need to be confirmed by Sequencing. We offers mutation screening method based on Illumina sequencing of target genes amplified from multi-dimensionally pooled templates representing 768 individuals per experiment.
