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Principle
- Thioglycolate Agar is used for the cultivation of aerobic as well as anaerobic microorganisms from pharmaceutical products. It is consisting of casein enzymic hydrolysate, L-cystine, dextrose, yeast extract, sodium chloride, sodium thioglycolate, resazurin, and agar.
- Casein enzymic hydrolysate and yeast extract provide nitrogen, carbon, vitamins, and other growth factors. Dextrose is an energy source. L-Cystine lowers the oxidation-reduction potential of the medium by removing oxygen to maintain a low Eh and prevent accumulation and toxicity of peroxides.
- Sodium thioglycolate neutralizes the antibacterial effect of preservatives and heavy metals in commercial products. Sodium chloride source of essential ions. Resazurin is an indicator, it is colorless in a reduced state and turns pink in oxidized state.
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