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Principle
- TAT Broth Base is an enrichment medium developed to isolate and cultivate microorganisms. TAT Broth Base conforms to the formula specified by US Pharmacopeia for use in Microbial Limit Tests.
- It is used for enrichment and further isolation and cultivation of gram-negative bacteria in cosmetics, topical drugs, and in the sterility testing of viscous or gelatinous substances.
- It consists of tryptone and azolectin. tryptone provides nitrogen, vitamins, amino acids, and carbon. Azolectin neutralizes preservatives in cosmetics or pharmaceutical products and allows the bacteria to grow easily. The media is fortified with Tween 20 for complete neutralization of preservatives.
- Prepare serial dilutions of the sample to be tested from 10-1 to 10-6. Inoculate 1 gram (1 ml) sample and 1 ml of each dilution into 40 ml of TAT Broth and after incubation, subculture the growth on MacConkey Agar.
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Use: Recommended for the enrichment and further isolation and cultivation of gram-negative bacteria in cosmetics, topical drugs and in the sterility testing of viscous or gelatinous substances.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Light tan colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light amber color clear to slightly opalescent solution.
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pH
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7.2 ±0.2 at 25 ˚C (25.00 g/ l)
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