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Principle
- Stanier's Base medium is used for the cultivation of Nitrosomonas and Nitrobacter. Nitrosomonas is a genus of Gram-negative bacteria, belonging to the Betaproteobacteria. It is one of the five genera of ammonia-oxidizing bacteria and, as an obligate chemolithoautotroph, uses ammonia as an energy source and carbon dioxide as a carbon source in presence of oxygen.
- Stanier's medium is composed of magnesium Sulphate, Dipotassium phosphate, Iron sulfate, Calcium chloride, manganese chloride, and Sodium molybdate. These slats are essential or provide favorable conditions for the growth of Nitrosomonas and an additional supplement of calcium carbonate provides a carbon source.
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Nitrobacter is a genus comprising rod-shaped, gram-negative, and chemoautotrophic bacteria. Nitrobacter plays an important role in the nitrogen cycle by oxidizing nitrite into nitrate in soil and marine systems.
- Unlike plants, where electron transfer in photosynthesis provides the energy for carbon fixation, Nitrobacter uses energy from the oxidation of nitrite ions, NO2−, into nitrate ions, NO3−, to fulfill their energy needs. Nitrobacter fixes carbon dioxide via the Calvin cycle for its carbon requirements.
- Stanier's Base medium is composed of magnesium Sulphate, Dipotassium phosphate, Iron sulfate, Calcium chloride, Manganese chloride, and Sodium molybdate. These slats are essential for the growth of Nitrobacter in addition the media is supplemented with sodium nitrile, which acts as a nitrogen source.
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