Sanger sequencing is the first-generation DNA sequencing method. It is designed to determine the order of nucleotides in a piece of DNA (commonly less than 1000 bp in length). Sanger sequencing with 99.99% base accuracy is considered the “gold standard” for validating DNA sequences, including those already sequenced through next-generation sequencing NGS.
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Service type
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Unit
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Plasmid DNA Sequencing
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Reaction
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Plasmid DNA Isolation
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Sample
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PCR product sequencing- Purified
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Reaction
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Gel Extraction - PCR products
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Sample
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Colum Purification- PCR products
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Sample
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Primer Walking
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Base
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Ready To Run (Cycle sequenced products)
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96 Well Plates
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Quality check of sanger sequencing sample
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Well
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Amplification & Sequencing (≤ 1000bp)
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Amplicon
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Microsatellite Genotyping Ready To Run
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Well
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Microsatellite Genotyping Ready To Run
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96 Well Plates
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Bacterial Identification (16S rRNA gene sequencing)
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Sample
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Fungal Identification (ITS rRNA gene sequencing)
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Sample
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Algae Identification (18s rRNA GENE sequencing)
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Sample
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Yeast Identification (ITS rRNA gene sequencing)
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Sample
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Actinomycetes Identification (16S rRNA gene sequencing)
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Sample
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Archaea Identification (15S rRNA gene sequencing)
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Sample
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Fish Identification (COI)
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Sample
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