Salmonella Shigella Agar

 Principle

• Salmonella Shigella agar is composed of protease peptone, meat extract, lactose, bile salts, sodium citrate, sodium thio-sulfate, ferric citrate, neutral red, brilliant green, and agar.
• Proteose peptone and meat extract provide essential growth nutrients. Lactose is a fermentable carbohydrate. Brilliant green, bile salts, and thiosulphate provide selective and inhibit gram-positive and coliform organisms.
• Certain organisms reduce sodium thiosulphate to sulfite and H₂S gas. Sodium thiosulfate and ferric citrate allow the detection of hydrogen sulfide by the production of colonies with black centers.
• Neutral red and brilliant green are present as pH indicators.

 Use: Recommended for the selective isolation and determination of Salmonella and some Shigella sp. from clinical and non-clinical samples.