Principle
- Sabouraud dextrose broth is used for the cultivation of yeasts, molds, and aciduric bacteria.
- It is recommended by USP as a medium for microbial limit testing of pharmaceutical products and raw materials used in pharmaceutical industries.
- The medium is prepared in accordance with the harmonized principles of USP. Medium is consisting of meat and casein peptone (1:1) and dextrose monohydrate.
- The meat and casein peptone provide carbonaceous, nitrogenous compounds, long-chain amino acids, vitamins, and other essential growth nutrients in addition to that the dextrose monohydrate serves as an energy source.
- The high concentration of dextrose and low pH of the medium favor the growth of yeasts and molds and inhibit other contaminating bacteria from pharmaceutical and clinical specimens.
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Use:
Recommended for the cultivation of yeasts, molds, and aciduric bacteria from pharmaceutical products in accordance with microbial limit testing by harmonized principles of USP.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Light beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Light yellow to medium amber, very slightly opalescent without significant precipitate
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pH
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5.6 ±0.2 at 25 ˚C (30.00 g/ l)
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