Principle
- Sabouraud dextrose agar is used for cultivation of yeasts, molds and aciduric bacteria. It is also recommended by IP as a medium for microbial limit testing of pharmaceutical products and raw material used in pharmaceutical industries.
- Medium is consisting of meat and casein peptone (1:1), dextrose monohydrate and agar.
- The meat and casein peptone provide carbonaceous, nitrogenous compounds, long chain amino acids, vitamins and other essential growth nutrients in addition to that the dextrose monohydrate serves as energy source.
- The high concentration of dextrose and low pH of medium favor the growth of yeasts and molds and inhibit other contaminating bacteria from pharmaceutical and clinical specimens.
- Agar is used as solidifying agent. Chloramphenicol inhibits a wide range of gram-positive and gram-negative bacteria making the medium selective for fungi.
Use:
Recommended for selective cultivation of yeasts and molds in accordance with Indian Pharmacopoeia, 2018.
|
PARAMETERS
|
STANDARD VALUES
|
|
PHYSICAL PARAMETERS:
|
|
Dehydrated Powder
|
Description
|
A fine, free flowing, hygroscopic powder.
|
|
Color
|
Lightbeige colored powder.
|
|
Solution
|
Solubility
|
Soluble in Distilled / de-ionized water at 50˚ C
|
|
Color
|
Light yellow to medium amber, veryslightly opalescent
|
|
pH
|
5.6±0.2 at 25˚C (65.00 g/ l)
|
|
Agar Plates
|
Solidification
|
40 ˚C
|
|
Color
|
Light yellow to medium amber, veryslightly opalescent gel
|
