Sabouraud Dextrose Agar

 Principle

• Sabouraud dextrose agar is used for the cultivation of yeasts, molds, and aciduric bacteria. It is also recommended by USP, IP, JP, and EP as a medium for microbial limit testing of pharmaceutical products and raw materials used in pharmaceutical industries.
• The medium is prepared in accordance with the harmonized principles of USP/EP/IP/JP. Medium is consisting of meat and casein peptone (1:1), dextrose monohydrate, and agar.
• The meat and casein peptone provide carbonaceous, nitrogenous compounds, long-chain amino acids, vitamins, and other essential growth nutrients in addition to that the dextrose monohydrate serves as energy source.
• The high concentration of dextrose and low pH of the medium favor the growth of yeasts and molds and inhibit other contaminating bacteria from pharmaceutical and clinical specimens. Agar is used as a solidifying agent.

 Use: Recommended for the cultivation of yeasts, molds, and aciduric bacteria from pharmaceutical products in accordance with the microbial limit testing by harmonized principles of USP/EP/BP/IP.