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Principle
- Rose Bengal chloramphenicol agar base is composed of mycological peptone, dextrose, potassium dihydrogen phosphate, magnesium sulfate, rose Bengal, chloramphenicol, and agar.
- Mycological peptone provides carbon, nitrogen substances, long-chain amino acids, vitamins, and other essential growth nutrients. Dextrose is an energy source. Monopotassium phosphate acts as a buffering agent.
- Magnesium sulfate provides ions for metabolic reactions. Rose Bengal is a selective agent, that inhibits bacterial growth and prevents over growth of fastidious fungi and aids in the isolation of slow-growing fungi.
- It also reduces the spreading of molds and controls the size and height of mold colonies such as Rhizopus species. The rose Bengal is absorbed by the yeast and molds and helps in identification and enumeration.
- Agar is the solidifying agent. Chloramphenicol has an inhibitory action on gram-negative bacteria.
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