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Principle
- Rose Bengal agar base is composed of soya peptone, dextrose, monopotassium phosphate, magnesium sulfate, rose bengal, and agar.
- Soya peptone provides nitrogen and amino acids and other trace nutrients required for the growth of microorganisms. Dextrose is an energy source. Monopotassium phosphate acts as a buffering agent.
- Magnesium sulfate provides ions for metabolic reactions. Rose Bengal is a selective agent, that inhibits bacterial growth and prevents the overgrowth of fastidious fungi, and aids in the isolation of slow-growing fungi.
- The rose Bengal is absorbed by the yeast and molds and helps in identification and enumeration. Agar is the solidifying agent. The media can be fortified with chloramphenicol, which inhibits bacteria.
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