Robertson Cooked Meat Medium (Cooked Meat Medium)

 Principle

• Robertson cooked meat medium is composed of special peptone (equivalent to beef heart, solids), protease peptone, dextrose, and sodium chloride.
• Special peptone and protease peptone provide nitrogen, amino acids, and essential trace nutrients required for the growth of anaerobic microorganisms. Dextrose in low concentration acts as a carbon and energy source.
• Sodium chloride maintains osmatic balance. The special peptone contains sulfhydryl group proteins; these SH-group proteins provide favorable conditions for anaerobic organisms due to reducing potential.
• In these mediums, the cooked meat particles are preferred because they provide desaturated proteins that contain easily accessible SH-groups, and impart a reducing effect.
• The dextrose allows quick and luxurious growth of anaerobic bacteria. In this medium the growth is indicated by turbidity or bubble formation and blackening and disintegration of the meat particles indicate proteolysis.

 Use: Recommended for cultivation and maintenance of aerobes and anaerobes microorganisms. Media is also used for the cultivation of pathogenic Clostridia from clinical, food, and water samples.