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Principle
- Reinforced medium for clostridia is used for enumerating and cultivating Clostridia. It is also recommended by USP, IP, and EP for microbial limit testing of pharmaceutical products and raw material used in pharmaceutical industries. Media is composed of meat extract (equivalent to beef extract), peptone, yeast extract, soluble starch, glucose monohydrate, cysteine hydrochloride, sodium chloride, sodium acetate and a small quantity of agar.
- Meat extract, peptone, yeast extract, starch, and cysteine hydrochloride provide nutrients and co-factors required for the growth of Clostridia. In addition to that cysteine hydrochloride acts as a reducing agent and soluble starch absorbs toxic metabolites from the medium. Glucose monohydrate is included in the medium as an energy source.
- Sodium chloride maintains osmotic equilibrium. Sodium acetate acts as a buffering agent. Less quantity of agar makes the medium semi-solid and helps in maintaining anaerobic conditions.
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