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- Estimation of copy number of transgene, relative quantification based on housekeeping gene expression data.
- Per gene Triplicate reactions to normalize the estimated copy.
Real-time PCR -
Real-time PCR (Polymerase Chain Reaction) is a laboratory technique used to amplify and quantify DNA or RNA molecules in real time. It is commonly used in various applications, including gene expression analysis, pathogen detection, and genetic testing. Some common applications of real-time PCR include:
- Gene Expression Analysis: Real-time PCR is widely used to measure gene expression levels. It enables researchers to quantify the amount of RNA transcripts produced by specific genes, allowing for the investigation of gene regulation, developmental processes, disease mechanisms, and drug responses.
- Pathogen Detection: Real-time PCR is employed for the rapid and sensitive detection of pathogens, including bacteria, viruses, and fungi. It can be used in diagnostic testing for infectious diseases, food safety monitoring, and environmental surveillance.
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Genetic Testing: Real-time PCR is utilized in genetic testing to identify and analyze specific DNA mutations or genetic variations associated with inherited disorders, predisposition to diseases, or pharmacogenetic profiling. It can be used in clinical diagnostics, personalized medicine, and forensics.
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Microbial Load Quantification: Real-time PCR allows for the quantification of microbial load in various samples, such as environmental samples, water quality testing, and food testing. It can provide valuable information about the abundance and dynamics of microbial populations.
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Copy Number Variation Analysis: Real-time PCR can be used to investigate copy number variations (CNVs) in the genome. CNVs are structural variations where sections of the DNA sequence are duplicated or deleted. Real-time PCR enables researchers to quantify the copy number of specific DNA regions and study their association with diseases or phenotypic variations.
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Allelic Discrimination: Real-time PCR can be employed for allelic discrimination to determine the presence or absence of specific genetic variations, such as single nucleotide polymorphisms (SNPs). It is widely used in genetic research, disease association studies, and pharmacogenetics.
These are just a few examples of the applications of real-time PCR. The technique's versatility and sensitivity make it a valuable tool in various scientific and diagnostic fields.
