Rappaport Vassiliadis Salmonella Enrichment Broth

 Principle

• The Rappaport Vassiliadis Salmonella Enrichment broth is the modification of the R10 medium (Rappaport et.al. 1956) or RV broth (Vassiliadis et.al. 1978), designed by Van Schothorst et al. (1987).
• Recommended by USP and JP for microbial limit testing medium for pharmaceutical products, especially for Salmonella species. The media is composed of soya peptone, magnesium chloride, sodium chloride, dipotassium phosphate, potassium dihydrogen phosphate, and malachite green.
• Soya peptone serves as carbon and nitrogen sources for growth requirements. Magnesium chloride raises the osmotic pressure in the medium. The malachite green and magnesium chloride together act as selective agents and inhibit the growth of other organisms than Salmonellae.
• The low pH of the medium (5.2±0.2 at 25°C), combined with the presence of malachite green and magnesium chloride, provides selectivity to highly resistant Salmonella spp. The phosphate buffers the medium.

 Use: Recommended for selective enrichment of Salmonella species from pharmaceutical products in accordance with the microbial limit testing by harmonized principles of USP/JP.