Principle
- The Rappaport Vassiliadis Medium is the modification of the R10 medium (Rappaport et.al. 1956) or RV broth (Vassiliadis et.al. 1978). The media is composed of soya peptone, magnesium chloride, sodium chloride, dipotassium phosphate, potassium dihydrogen phosphate, and malachite green.
- Soya peptone serves as carbon and nitrogen sources for growth requirements. Magnesium chloride raises the osmotic pressure in the medium. The malachite green and magnesium chloride together act as selective agents and inhibit the growth of other organisms than Salmonellae.
- The low pH of the medium (5.2±0.2 at 25°C), combined with the presence of malachite green and magnesium chloride, provides selectivity to highly resistant Salmonella spp. The phosphate buffers the medium.
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Use: Recommended for selective enrichment of Salmonella species, based on its ability to multiply selectively at high osmotic pressure, low pH, and at 43°C, with modest nutritional requirements.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Pale yellow with blue tinge colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Greenish blue colored opalescent solution
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pH
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5.2 ±0.2 at 25 ˚C (30.00 g/ l)
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