Affinity purification of protein
Protein A/G affinity is a type of affinity chromatography that can be applied to antibodies.
Protein A/G 4FF (Fast Flow) is a highly cross-linked 4% agarose gel matrix that is used for immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) studies, as well as for antibody immobilization and other related research.
Protein A/G FF is a high-density orientation of Recombinant Protein A/G coupled to highly crosslinked agarose gel microspheres with higher antibody binding capacity and lower protein non-specific adsorption. The elution conditions are more uniform, and the antibody with a purity >90% can be isolated from the serum sample in one step .
| Size |
5 x 1ml / 1 x 5ml / 5 x 5ml |
| Product Category |
Affinity Purification |
| Matrix |
Highly Cross-Linked 4% Agarose Beads |
| Ligand |
Recombinant Protein A/G |
| Binding Capacity |
10-15mg human lgG/ml |
| Bead Size |
45-165um |
| Pressure/Flow |
0.3 MPa, 3 bar |
| pH Stability |
3-10 |
| Storage Buffer |
1x PBS containing 20% Ethanol |
| Storage/Stability |
2-8°C/2 years |
| Shipping |
Gel Packs |
