Protein A Beads 4FF- affinity purification of protein
Protein A Beads 4FF (Fast Flow) is a highly cross-linked 4% agarose gel matrix that allows the purification of monoclonal and polyclonal antibodies at relatively high flow rates and is widely accepted by industrial customers.
Protein A FF is a versatile affinity chromatography medium for the isolation and purification of monoclonal antibodies, polyclonal antibodies, or Fc-fusion tags. Protein A is a cell wall protein isolated from S. aureus that binds mammalian IgG primarily through the Fc fragment but does not bind to dog lgG and does not bind human lgM, lgD, and IgA. Protein A and protein G have different immunoglobulin binding abilities from different sources and subclasses. Natural Protein A has five IgG binding regions and some regions of unknown function. Recombinant protein A removes binding sites with albumin and cell surface and contains only five IgG binding regions, reducing non-specific adsorption.
Size |
5ml / 10ml |
Product Category |
Affinity Purification |
Matrix |
Highly Cross-Linked 4% Agarose Beads |
Ligand |
Recombinant Protein A |
Binding Capacity |
>40mg human lgG/ml |
Bead Size |
45-165um |
Pressure/Flow |
0.3 MPa, 3 bar |
pH Stability |
3-10 |
Storage Buffer |
1x PBS containing 20% Ethanol |
Storage/Stability |
2-8°C/2 years |
Shipping |
Gel Packs |