Primer Walking on Single DNA stands
DNA sequencing will start with two universal primers & continuously use primers derived from the end of the newly determined DNA sequences until the overlapping region of both stands is determined. This method is suitable for sequencing of constructs with known insert sequences.
Description
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Primer walking single strand- Primer design, synthesis and sequencing, final alignment of data (Per Base)
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Primer walking single strand for AT or GC rich sequences-Primer design, synthesis and sequencing, final alignment of data (Per Base)
