Plant Protease Phosphatase Inhibitor [50x]

Therefore, adding appropriate protease, phosphatase and other inhibitors to the extract is to prevent degradation and demodification This product is a mixture of protease and phosphatase inhibitors used in the extraction of plant cells or tissue proteins. It contains a broad spectrum of serine, cysteine, and acid protease inhibitors/aminopeptidase inhibitors, as well as serine/threonine, tyrosine, acid, and alkaline phosphatases inhibitors. If used to detect metalloproteinase activity, EDTA should not be added. Add the protease phosphatase inhibitor to the lysis solution at a ratio of 1:50 to extract plant cell or tissue protein by adding it to the lysis solution, and effectively inhibit protein degradation.

Scope of application: inhibit various proteases in plant cells or tissue extracts (such as serine proteases, aminopeptidases, cysteine proteases, threonine proteases) Acid and aspartic proteases, metalloproteinases, etc.) and phosphatases (such as serine/threonine, tyrosine, acid, and alkaline phosphatase, etc.). Suitable for Western Blot and immunoprecipitation detection of phosphorylated protein, protein kinase activity determination, etc.