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Principle
- Pikovskayas Broth was modified by Sundara Rao and Sinha (1963) for the cultivation of phosphate-solubilizing bacteria from soil. It is composed of yeast extract, dextrose, calcium phosphate, ammonium sulfate, potassium chloride, magnesium sulphate, manganese sulphate, and ferrous sulphate.
- Yeast extract provides nitrogen, carbon, vitamins, and other nutrients necessary to support bacterial growth. Dextrose acts as an energy source.
- Different salts and yeast extract supports the growth of organisms and enhances phosphate solubilization.
- Calcium phosphate is the source of inorganic phosphate.
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