|
Principle
- Pikovskayas Agar was modified by Sundara Rao and Sinha (1963) for the detection of phosphate-solubilizing bacteria from soil. It is composed of yeast extract, dextrose, calcium phosphate, ammonium sulphate, potassium chloride, magnesium sulphate, manganese sulphate, ferrous sulphate and agar.
- Yeast extract provides nitrogen, carbon, vitamins and other nutrients necessary to support bacterial growth.
- Dextrose acts as an energy source. Different salts and yeast extract supports the growth of organisms and enhances phosphate solubilization. Agar is the solidifying agent. Calcium phosphate is inorganic phosphate, that solubilizes to form a clear zone around the colonies.
|
