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Principle
- Phenylalanine agar is composed of yeast extract, dipotassium phosphate, sodium chloride, DL-phenylalanine, and agar.
- Yeast extract provides nitrogen, carbon, vitamins, and other necessary growth factors. Dipotassium is a buffering agent. Sodium chloride maintains osmatic balance.
- DL-phenylalanine is a substrate for Phenylalanine deaminase, deaminating DL-phenylalanine to phenyl pyruvic acid. The synthesized phenylpyruvic acid is detected by the addition of a ferric chloride reagent.
- The ferric chloride reacts with phenyl pyruvic acid to form a green color complex. Agar is the solidifying agent.
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