PGF2a (Prostaglandin F2 Alpha) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The micro-plate provided in this kit has been pre-coated with Prostaglandin F2 Alpha (PGF2a) protein. Standards or samples are then added to the appropriate micro-plate wells with a biotin-conjugated antibody specific to Prostaglandin F2 Alpha (PGF2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Prostaglandin F2 Alpha (PGF2a) in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit Components:
1) Pre-coated Microplate [12 strips x 8 wells]
2) 100x Biotinylated Conjugate [120ul]
3) Biotinylated Conjugate Diluent [10ml]
4) 100x Streptavidin-HRP [120ul]
5) HRP Diluent [12ml]
6) TMB Substrate Solution [9ml]
7) 25x Wash Buffer [20ml]
8) Stop reagent [6ml]
9) Lyophilized Standard [2]
10) Standard Diluent Buffer [20ml]
11) Plate Sealers [2]
12) User Manual [1]