Pfu Polymerase Mix [2x]

2x Pfu DNA Polymerase Mastermix is a premixed; ready-to-use solution containing Pfu DNA Polymerase; dNTPs; MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR; only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors; miscalculation and contamination.

Pfu DNA polymerase; derived from the hyperthermophilic archae Pyrococcus furiosus; has been shown to exhibit superior thermostability and proofreading properties compared to other polymerases. Unlike Taq polymerase; highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products; which are ideal for cloning into blunt-ended vectors. Use Pfu polymerase for techniques that require high-fidelity DNA synthesis.