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Principle
- Perfringens Agar (O.P.S.P.) is based on the formula developed by Handford (1974) and the media is composed of tryptone, soya peptone, yeast extract, liver extract, ferric ammonium citrate, sodium metabisulphite, tris base and agar.
- Tryptone, soya peptone, liver extract, and yeast extract serve as sources of carbon, nitrogen, amino acids, and other necessary elements for growth. Sodium metabisulphite and ferric ammonium citrate are an indicators for hydrogen sulfate production or sulfate reduction.
- The tris provide buffering capacity. The media is fortified with antibiotics like polymyxin B, sulphadiazine and oleandomycin to increase the selection of medium and to inhibit sulfite-reducing bacteria other than C. perfringenes.
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