|
Principle
- Peptone water is composed of peptone and sodium chloride. Peptone serves as the simplest source of nitrogen, carbon, and other growth factors such as vitamins. Sodium chloride maintains the osmotic balance.
- Peptone is rich in tryptophan and used for studying indole production. The production of indole can be detected by using Kovacs or Ehlrich reagents.
- Peptone water is also utilized as a base for carbohydrate fermentation studies with the addition of sugar and indicators such as phenol red, bromocresol purple, or bromothymol blue.
|
|
Use: Recommended for the cultivation of non-fastidious organism, for studying carbohydrate fermentation patterns, and for performing the indole test.
|
|
PARAMETERS
|
STANDARD VALUES
|
|
PHYSICAL PARAMETERS:
|
|
Dehydrated Powder
|
Description
|
A fine, free flowing, hygroscopic powder.
|
|
Color
|
Beige colored powder.
|
|
Solution
|
Solubility
|
Soluble in Distilled / de-ionized water at 50˚ C
|
|
Color
|
Light amber color clear solution
|
|
pH
|
7.2±0.2 at 25˚C (15.00 g/ l)
|
|
