Principle
- NB is a basic media and is commonly used for the cultivation, enrichment, enumeration, prolonged maintenance, and storage of a wide variety of microbes.
- The nutrient broth is widely used as non-selective microbial media for routine microbiology analysis and cultivation of non-fastidious organisms.
- The nutrient broth is ideal microbial media for teaching, demonstration, and routine analysis.
- Nutrient Broth (NB) is a simple medium composed of peptone, meat extract (Equivalent to beef extract), yeast extract, and sodium chloride.
- Peptone, meat extract, and yeast extract provide ample amounts of the carbon and nitrogen essential and non-essential amino acids, along with vitamins and trace minerals. Sodium chloride maintains the osmotic equilibrium of the medium.
Use:
Recommended for the refinement of non-fastidious microorganisms from clinical and non-clinical samples. The nutrient broth is ideal microbial media for teaching, demonstration, and routine analysis.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Beige-colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Pale yellow colored clear to slightly opalescent solution.
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pH
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7.4 ±0.2 at 25 ˚C (13.00 g/ l)
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