Ni-NTA Magnetic Beads

Overview

BOINEER’s Ni-NTA Magnetic Beads are coated with high-density of Nickel-nitrilotriacetic acid (Ni-NTA) on the surface of Silica Magnetic Beads. Depending on the size, Biotin Magnetic Beads are available as micrometer-sized AccuBead™ (1 - 5 μm) and nanometer-sized AccuNanoBead™ (400 nm). All magnetic beads developed by BIONEER have a pure magnetite (iron(Ⅱ, Ⅱ) oxide, Fe3O4) core. Compared to other beads that use polymer cores, BIONEER’s magnetic beads have higher magnetic saturation, allowing them to be collected more quickly by a magnet. This enables faster separation and purification of target biomolecules. Additionally, BIONEER’s technology allows for more functional groups to be attached to the surface, increasing the efficiency of experiments by capturing more target biomolecules with the same volume as magnetic beads.
Histidine residues in the His tag have a strong affinity for nickel ions, and Nitrilotriacetic acid (amino-polycarboxylic acid, N(CH₂CO₂H)₃) works as a chelating agent. Ni-NTA Magnetic beads can purify 6x His-tagged proteins. Using the MagListo™ Magnetic Separation Rack (TM-1000, TM-1010, TM-1011, TM-1020, TM-1030) or other magnetic separation racks, biomolecules attached to the beads can be easily purified by magnetic force.

Features & Benefits

• A high magnetic density of 80.8 emu/g derived from a pure magnetite(Fe₃O₄) core

• Rapid magnetic capture within 5 seconds with a high magnetic density

• A high binding capacity of target molecules because of highly dense functional groups and smaller size
• Microplastics-free as no plastic cores like polystyrene

Applications

• Isolating and purifying Histidine-tagged peptides and proteins from samples