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Principle
- Mycoplasma agar base was described by Morton, Smith, and Leberman (1951) for the isolation and cultivation of Mycoplasma species. It is composed of meat heart infusion form (equivalent to beef heart infusion form), peptone, sodium chloride, and agar.
- Meat heart infusion form and peptone provide nitrogen, amino acids, carbon vitamins, and essential trace nutrients for the growth of microorganisms. Sodium chloride maintains osmatic balance.
- Agar is the solidifying agent. For mycoplasma cultivation the media can be enriched with horse serum and media selectivity can be increased by the addition of thallous acetate (25 mg/l) and penicillin G (20000IU/L). Mycoplasma are widely distributed microorganisms with huge diversity.
- Mycoplasma belongs to aerobic or facultatively anaerobic, anaerobic saprophytic and some are pathogenic.
- Therefore, the incubation temperature and period are various from species to species. For most of the mycoplasms species, the cultures are incubated in an anaerobic atmosphere containing 5-10% carbon dioxide and examined for 24 hours to 3 weeks.
- Pleuropneumonia-Like Organisms (PPLO) Mycoplasmataceae, colonies are round with a dense center, resembling a “fried egg” on agar.
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