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Principle
- Mueller Hinton agar with 2% glucose and methylene blue is the modification of the formula recommended by Mueller and Hinton (1941) for the primary isolation of Neisseria species. Commonly it is used for performing antibiotic susceptibility tests using a disc diffusion assay and due to its susceptibility to microorganisms and high reproducibility; WHO Committee recommended it for standardization of susceptibility testing.
- Media is simple and composed of meat infusion form (equivalent to beef infusion form), Casamino acids, starch, glucose, methylene blue, and agar. Meat Infusion is from and casamino acids provides nitrogen vitamins, amino acids, minerals, carbon and other nutrients to support the growth of microorganisms.
- While starch absorbs any toxic metabolites produced during microbial growth and autoclaving undergoes hydrolysis and liberates a small amount of dextrose, which acts as the source of energy. The additional glucose serves as an energy source for fungal cultures while Methylene blue enhances zone edge definition. Agar is the solidifying agent.
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