Principle
- Mueller Hinton agar duplicates the formula recommended by Mueller and Hinton (1941) for the primary isolation of Neisseria species. Commonly it is used for performing antibiotic susceptibility tests using a disc diffusion assay and due to its susceptibility to microorganisms and high reproducibility; the WHO Committee recommended it for standardization of susceptibility testing.
- Media is simple and composed of meat infusion form (the equivalent of beef infusion form), Casamino acids, starch, and agar. Meat Infusion from casamino acids provides nitrogen vitamins, amino acids, minerals, carbon, and other nutrients to support the growth of microorganisms.
- While starch absorbs any toxic metabolites produced during microbial growth and autoclaving undergoes hydrolysis and liberates a small amount of dextrose, which acts as the source of energy. Agar is the solidifying agent.
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