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Principle
- Mueller Kauffman Tetrathionate Broth Base is used for the isolation of Salmonella species. The media is composed of casein peptone, soya peptone, sodium chloride, calcium carbonate, sodium thiosulphate, and ox bile.
- Casein and soya peptone provide nutrients essential for the growth of microorganisms. Sodium chloride maintains osmotic equilibrium and calcium carbonate neutralizes the acidic byproducts of tetrathionate decomposition. After sterilization potassium iodide is added which reacts with sodium thiosulphate to form thiosulphate and tetrathionate.
- The formed thiosulphate and tetrathionate suppress the growth of coliform organisms and provide selectivity to the medium. Ox bile and brilliant green as selective agents to suppress bacteria such as Proteus species.
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Use: Recommended for selective enrichment broth for isolation of Salmonella.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Light beige colored powder.
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Solutions
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Green colored opalescent solution
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Complete medium with added iodine solution and Brilliant green solution
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Color
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Light green opalescent with white precipitate, on standing the precipitate settles down.
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