Mueller Hinton Broth

 Principle

• Mueller Hinton Medium duplicates the formula recommended by Mueller and Hinton (1941) for the primary isolation of Neisseria species. Currently its widely used for performing antibiotic susceptibility tests using the tube dilution method.
• Media is simple and composed of meat infusion form (equivalent to beef infusion form), Casamino acids, and starch. Infusion from Meat and Casamino acids provides nitrogen vitamins, amino acids, minerals, carbon, and other nutrients to support the growth of microorganisms.
• While starch absorbs any toxic metabolites produced during microbial growth and autoclaving undergoes hydrolysis and liberates small amount of dextrose, which acts as a source of energy.

 Use: Recommended for antimicrobial susceptibility testing of rapidly growing aerobic and facultative anaerobic microbes by the tube dilution method.