MTT Cytotoxicity Assay - Cell Detection Reagent
MTT Cytotoxicity Assay provides a simple and accurate method to quantify cell proliferation and viability.
The assay is based on the conversion of water-soluble MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan. Dead cells, on the other hand, lose this ability and therefore show no signal. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance at OD 590 nm is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high throughput method for measuring cell proliferation, cell viability, and cytotoxicity.
Size |
500 Assays |
Product Category |
Cell Detection |
Kit Components |
1. MTT Solution 2. Formazan Solvent |
Storage/Stability |
-20°C/1 year |
Shipping |
Gel Packs |