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Principle
- Modified pseudomonas selective agar with cetrimide is composed of skimmed milk powder, peptone, sodium chloride, yeast extract, cetrimide, and agar. Skimmed milk is the source of casein, hydrolyzed by Pseudomonas to form yellow to green diffusible pigmented colonies.
- Peptone provides nitrogen and amino acids and necessary nutrients. Yeast extract is the source of carbon, nitrogen, vitamins, and required growth factors. Sodium chloride essential ions. Cetrimide acts as a quaternary ammonium, a cationic detergent that causes the release of nitrogen and phosphorus from bacterial cells other than P. aeruginosa.
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