Membrane Regeneration Solution

Using a membrane regeneration solution can perform multiple Western Blot detection on the same membrane. At room temperature, soak the used membrane in membrane regeneration solution for 10-60 Minutes, the antibody bound on the membrane can be removed without affecting the antigen protein. Subsequently, a different antibody can be used for the next round of the Western Blot experiment. It is suitable for multiple detection of different proteins from a small number of samples. Even if the sample amount is not scarce, using this strategy to detect the protein on the same membrane multiple times, can save time-consuming steps such as administration treatment, protein electrophoresis, and membrane transfer. And it can eliminate the errors caused by reloading, making the comparability stronger.

The features of Western Blot membrane regeneration solution are: non-toxic, tasteless, and harmless, used at room temperature, and stored at room temperature; it can perform Western blotting on the same membrane more than 5 times Blot detection.

Application: Remove the antibody bound on the nitrocellulose membrane or PVDF membrane without affecting the antigen protein.