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Principle
- Mannitol salt broth is a medium used for the detection and enumeration of Staphylococci. Mannitol salt broth is composed of a pancreatic digest of casein, peptic digest of animal tissue, and meat extract (equivalent to beef extract) as sources of carbon, nitrogen, vitamins, and minerals.
- Sodium chloride, in high concentration (7.5%), acts as selective agent, allowing only to grow the Staphylococci and halophilic Enterobacteria and inhibit other bacteria.
- Phenol red is the pH indicator. D-Mannitol is the carbohydrate source. Mannitol fermentation with an accumulation of acid products, is detected by the phenol red indicator which turns yellow.
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