Principle
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MacConkey Broth is a modification of the original bile salt broth recommended by MacConkey (1901) and Childs and Allen (1953).
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MacConkey broth is prepared in accordance with the harmonized principles of USP/EP/IP and used for the identification of coliforms in pharmaceutical testing and microbial limit testing of pharmaceutical products and raw materials used in pharmaceutical industries.
- It consists of a pancreatic digest of gelatin, lactose monohydrate, dehydrated ox-bile, and bromocresol purple.
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The pancreatic digest of gelatin provides amino acids and other growth factors. Lactose monohydrate is a carbon energy source for gram-negative lactose-fermenting bacilli.
- Ox bile inhibits the growth of gram-positive organisms. Bromcresol purple acts as the pH indicator. Bromocresol purple is highly sensitive in recording pH variation and changes its color from purple to yellow below pH 6.8.
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Use:
Recommended for identification of coliforms in pharmaceutical testing and microbial limit testing of pharmaceutical products and raw materials used in pharmaceutical industries.
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PARAMETERS
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STANDARD VALUES
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Light beige colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Purple, clear opalescent solution
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pH
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7.3 ±0.2 at 25 ˚C (35.00 g/ l)
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