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Principle
- MacConkey broth is composed of peptone, protease peptone, lactose, bile salt, sodium chloride, and neutral red.
- Peptone and protease peptone provide nitrogen and other nutrients necessary for the growth of microorganisms. Lactose is a carbon source and plays an important role in selection of lactose-fermenting microbes.
- Bile salt is a selective agent, inhibits the growth of gram-positive organisms. Neutral red is a pH indicator dye. When lactose is fermented, acid and turns medium pink to yellow.
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Use: Recommended for the enrichment and enumeration of lactose fermenting coliforms from pharmaceutical, clinical, and non-clinical samples, dairy, food, and water samples.
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free flowing, hygroscopic powder.
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Color
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Light pink colored powder.
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Solution
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Solubility
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Soluble in Distilled / de-ionized water at 50˚ C
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Color
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Red colored opalescent clear solution
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pH
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7.5 ±0.2 at 25 ˚C (80.00 g/ l)
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