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Principle
- Lowenstein-Jensen medium base is used for the isolation of acid-fast microorganisms like Mycobacterium species.
- The base medium is composed of L-asparagine, monopotassium phosphate, magnesium sulfate, magnesium citrate, potato starch soluble, and malachite green.
- L-asparagine is the source of nitrogen. Mono-potassium phosphate and magnesium sulfate enhance organism growth and act as buffers. Potato starch is the source of carbon and absorbs toxic metabolites, malachite green is used to prevent the growth of most contaminants and encourages the growth of mycobacteria.
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Use: Recommended for isolation, cultivation, and differentiation of mycobacteria.
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PHYSICAL PARAMETERS:
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Dehydrated Powder
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Description
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A fine, free-flowing, hygroscopic powder.
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Color
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Blue to greenish blue colored powder.
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Solution
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Solubility
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The mixture of sterile basal medium and whole egg emulsion, yields pale bluish-green colored
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Color
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Light yellowish to creamy colored opalescent
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pH
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6.9 ±0.2 at 25 ˚C (37.30 g/ l)
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Agar Plates
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Solidification
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40 ˚C
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Color
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The mixture of sterile basal medium and whole egg emulsion coagulates to yield pale bluish-green colored, opaque smooth gel
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